Skip to main content

Table 2 Reference and target gene efficiency determination and amplicon melting points, r 2 values are regression coefficients for the curves

From: Identification of appropriate reference genes for qPCR studies in Staphylococcus pseudintermedius and preliminary assessment of icaA gene expression in biofilm-embedded bacteria

Candidate reference genes Slope of the curve r2on the slope PCR efficiency Melting temperature (°C)
proC −3.78 0.997 1.84 80.0
gyrB −3.85 0.998 1.82 79.5
rplD −3.86 0.998 1.82 75.5
rho −3.68 0.997 1.87 81.0
rpoA −3.89 0.996 1.81 76.5
ftsZ −3.85 0.998 1.82 79.0
recA −3.83 0.998 1.82 81.0
sodA −3.97 0.996 1.79 79.5
Target gene     
icaA −3.92 0.995 1.80 80.5