Visualization of staphylococcal biofilms grown in the presence of human plasma by CLSM and SEM. A-B) CLSM images biofilms of a methicillin-resistant (MRSA-3) and methicillin susceptible (UAMS-1) isolates of S. aureus grown overnight on coverslips in media supplemented with or without human plasma (10% Plasma). Biofilms were stained with a bacterial and a biofilm matrix stain to visualize the bacterial cells and extracellular polymeric matrix, respectively. Images were captured at 20X magnification. C-D) SEM analysis of biofilms of the clinical isolates listed above, following exposure to human plasma. Images were captured at 40,000X. Inlayed size bars represent 200 nm.