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Table 1 S. aureus biofilm formation is more enhanced when plasma is part of the growth medium, compared to coating plates with plasma prior to biofilm formation

From: Human plasma enhances the expression of Staphylococcal microbial surface components recognizing adhesive matrix molecules promoting biofilm formation and increases antimicrobial tolerance In Vitro

Strain Percent plasma Plasma coated Plasma in medium P-value
   OD570 (mean + SD)* OD570 (mean + SD)  
MSSA UAMS-1     
  0 0.61±0.34 0.87±0.31  
  1 0.81±0.41 1.63±0.48 0.15
  5 0.56±0.33 1.4±0.58 0.17
  10 0.25±0.08 2.4±0.46 0.01
  25 0.3±0.2 1.95±0.58 0.02
  50 0.32±0.17 0.83±0.76 0.22
MRSA 1     
  0 0.36±0.13 0.38±0.18  
  1 0.28±0.06 0.77±0.26 0.06
  5 0.67±0.47 1.08±0.49 0.22
  10 0.56±0.44 1.44±0.33 0.03
  25 0.58±0.27 1.22±0.3 0.08
  50 0.38±0.21 0.67±0.56 0.33
MRSA 2     
  0 0.38 + 0.13 0.6 ± 0.19  
  1 0.57 + 0.02 2.26 + 0.17 0.04
  5 0.79 + 0.47 2.37 + 0.1 0.04
  10 0.7 + 0.58 2.82 + 0.04 0.03
  25 0.67 + 0.49 2.47 + 0.6 0.04
  50 0.5 + 0.25 1.3 + 0.99 0.24
MRSA 3     
  0 0.58 + 0.31 0.53 ± 0.14  
  1 0.5 + 0.07 2.46 + 0.57 0.04
  5 0.93 + 0.69 2.26 + 0.71 0.11
  10 0.63 + 0.16 2.18 + 0.68 0.04
  25 0.53 + 0.51 2.5 + 0.37 0.02
  50 0.36 + 0.16 1.84 + 1.0 0.14
  1. *96-well plates were coated with plasma for 24 hours, washed then biofilm was formed.
  2. Plasma coated compared to plasma in the growth medium. P-values generated via One-way ANOVA with a Dunnett’s post hoc test.
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