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Table 2 Increase in biofilm biomass measured by Crystal Violet method following incubation in plasma

From: Human plasma enhances the expression of Staphylococcal microbial surface components recognizing adhesive matrix molecules promoting biofilm formation and increases antimicrobial tolerance In Vitro

 

UAMS-1

MRSA-1

MRSA-2

MRSA-3

 

OD570±SD

%±SD

OD570±SD

%±SDs

OD570±SD

%±SD

OD570±SD

%±SD

0%

0.87±0.31

N/A

0.38±0.18

N/A

0.6 ± 0.19

N/A

0.53 ± 0.14

N/A

1%

1.63±0.48

87±29

0.77±0.26

102±34

2.26 ± 0.17*

277 ± 8ϯ

2.46 ± 0.57*

364 ± 23ϯ

5%

1.40±0.58

61±41

1.08±0.49*

189±45ϯ

2.37 ± 0.1*

295 ± 4ϯ

2.26 ± 0.71*

326 ± 31ϯ

10%

2.40±0.46

171±19

1.44±0.33*

279±23ϯ

2.82 ± 0.04*

370 ± 1ϯ

2.18 ± 0.68*

311 ± 31ϯ

25%

1.95±0.58

124±30

1.22±0.30*

221±25ϯ

2.47 ± 0.60*

317 ± 24ϯ

2.50 ± 0.37*

368 ± 15ϯ

50%

0.83±0.76

-5±92

0.67±0.56

76±84

1.30 ± 0.99

117 ± 76

1.84 ± 1.0

249 ± 54ϯ

  1. *P ≤ 0.05 vs. 0% plasma, One-way ANOVA with Dunnet’s post hoc test.
  2. ϯP ≤ 0.05 Percent increase in biofilm biomass (UAMS-1 vs. MRSA-1, MRSA-2, or MRSA-3) at comparable plasma percentages, One-way ANOVA with Dunnet’s post hoc test.
  3. Results are expressed as Mean OD570 ± standard deviation and percent change from 0% plasma ± SD.