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Table 2 Increase in biofilm biomass measured by Crystal Violet method following incubation in plasma

From: Human plasma enhances the expression of Staphylococcal microbial surface components recognizing adhesive matrix molecules promoting biofilm formation and increases antimicrobial tolerance In Vitro

  UAMS-1 MRSA-1 MRSA-2 MRSA-3
  OD570±SD %±SD OD570±SD %±SDs OD570±SD %±SD OD570±SD %±SD
0% 0.87±0.31 N/A 0.38±0.18 N/A 0.6 ± 0.19 N/A 0.53 ± 0.14 N/A
1% 1.63±0.48 87±29 0.77±0.26 102±34 2.26 ± 0.17* 277 ± 8ϯ 2.46 ± 0.57* 364 ± 23ϯ
5% 1.40±0.58 61±41 1.08±0.49* 189±45ϯ 2.37 ± 0.1* 295 ± 4ϯ 2.26 ± 0.71* 326 ± 31ϯ
10% 2.40±0.46 171±19 1.44±0.33* 279±23ϯ 2.82 ± 0.04* 370 ± 1ϯ 2.18 ± 0.68* 311 ± 31ϯ
25% 1.95±0.58 124±30 1.22±0.30* 221±25ϯ 2.47 ± 0.60* 317 ± 24ϯ 2.50 ± 0.37* 368 ± 15ϯ
50% 0.83±0.76 -5±92 0.67±0.56 76±84 1.30 ± 0.99 117 ± 76 1.84 ± 1.0 249 ± 54ϯ
  1. *P ≤ 0.05 vs. 0% plasma, One-way ANOVA with Dunnet’s post hoc test.
  2. ϯP ≤ 0.05 Percent increase in biofilm biomass (UAMS-1 vs. MRSA-1, MRSA-2, or MRSA-3) at comparable plasma percentages, One-way ANOVA with Dunnet’s post hoc test.
  3. Results are expressed as Mean OD570 ± standard deviation and percent change from 0% plasma ± SD.