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Table 3 10% Plasma alters MSCRAMM gene expression in a strain dependent fashion

From: Human plasma enhances the expression of Staphylococcal microbial surface components recognizing adhesive matrix molecules promoting biofilm formation and increases antimicrobial tolerance In Vitro

Strain

Gene

Fold increase in gene expression*

  

30 min

60 min

90 min

120 min

UAMS-1

Laminin binding protein (Eno)

1.62

2.06

2.76

3.39

 

Encoding elastin binding protein (ebps)

3.42

2.98

4.61

3.50

 

Fibrinogen binding protein (fib)

0.00

17.12

32.52

30.86

 

Clumping factor A (clfA)

2.08

3.57

6.03

4.43

 

Clumping factor B (clfB)

1.04

5.48

2.33

4.39

 

Fibronectin binding protein (fbnA)

11.22

4.28

5.25

1.96

 

Fibronectin binding protein (fbnB)

5.53

4.71

4.76

3.02

MRSA-1

Laminin binding protein (Eno)

1.51

2.15

2.85

2.51

 

Encoding elastin binding protein (ebps)

1.11

1.29

2.02

1.75

 

Fibrinogen binding protein (fib)

5.18

7.88

13.92

7.86

 

Clumping factor A (clfA)

2.88

2.82

3.69

4.10

 

Clumping factor B (clfB)

0.98

1.62

2.73

2.32

 

Fibronectin binding protein (fbnA)

32.23

46.84

30.30

2.79

 

Fibronectin binding protein (fbnB)

0.87

13.97

5.14

5.36

MRSA-2

Laminin binding protein (Eno)

1.06

3.53

3.83

3.63

 

Encoding elastin binding protein (ebps)

1.01

3.80

2.12

1.38

 

Fibrinogen binding protein (fib)

1.30

4.16

6.21

3.14

 

Clumping factor A (clfA)

4.08

3.13

3.90

2.62

 

Clumping factor B (clfB)

1.14

1.60

2.50

3.91

 

Fibronectin binding protein (fbnA)

2.59

7.14

4.89

2.00

 

Fibronectin binding protein (fbnB)

3.62

3.72

3.31

2.61

MRSA-3

Laminin binding protein (Eno)

1.31

1.86

3.96

2.95

 

Encoding elastin binding protein (ebps)

0.99

1.21

3.67

1.88

 

Fibrinogen binding protein (fib)

2.44

6.92

5.18

3.49

 

Clumping factor A (clfA)

1.82

1.73

5.56

2.73

 

Clumping factor B (clfB)

1.32

1.15

3.78

3.22

 

Fibronectin binding protein (fbnA)

2.57

2.08

4.26

5.30

 

Fibronectin binding protein (fbnB)

2.60

2.35

3.53

3.75

  1. *A greater than or equal to 3 fold increase was statistically significant (p<0.05) compared to baseline gene expression (t=0), and are indicated in boldface, One-way ANOVA with a Dunnet’s post hoc test.