No association found between the detection of either xenotropic murine leukemia virus-related virus or polytropic murine leukemia virus and chronic fatigue syndrome in a blinded, multi-site, prospective study by the establishment and use of the SolveCFS BioBank

Table 2 XMRV gag amino acid residues 8 to 120 in CFS, CFS positive control and healthy subjects

XMRV gag codon		024	031	032	034	035	036	045	046	047	048	049	050	051	059	060	105
Sample type	Sample ID	024	031	032	034	035	036	045	046	047	048	049	050	051	059	060	105
Published reference	VP62	S	K	K	R	W	V	T	F	N	V	G	W	P	G	V	P
Positive control reference	22Rv1	.	.	.	.	.	.	.	.	.	.	.	.	.	.	I	.
CFS positive control subject	G018	.	R	.	.	.	I								S	I	L
CFS subject	G026	.	R	.	.	.	I	.	.	.	.	.	.	.	S	I	L
CFS subject	G028	.	R	.	.	.	I	.	F/S	.	.	.	.	.	S	I	L
CFS subject	G035	.	R	.	.	.	I	.	.	.	.	.	.	.	S	I	L
CFS subject	G071	.	G	.	.	.	V	—	—	—	—	—	—	—	.	I	L
CFS subject	G091	.	R	.	R/C	.	I	.	.	.	.	.	.	.	S	I	L
Healthy subject	G053	.	R	.	.	.	I	.	.	.	.	.	.	.	S	I	L
Healthy subject	G046	P	R	K/E	.	Stop

Noted along the top are XMRV Gag amino acid residue positions where a difference was observed in one or more subject samples relative to the VP62 reference virus sequence. Single letter amino acid abbreviations are given for the VP62 reference virus. XMRV gag amino acid residues 8 to 120 in the 22Rv1 positive control reference virus, one CFS Positive Control Subject, five previously untested CFS Subjects and two Healthy Subjects are displayed. Residues that are identical to the VP62 reference are denoted by a dot (.), differences are denoted by a single letter amino acid abbreviation, and deletions are denoted by a dash (—). Healthy Subject G046 has a premature stop codon at residue 35. Also of note, Healthy Subject G053 has a single nucleotide deletion upstream of the Gag start codon (not shown).

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