Identification and validation of quantitative real-time reverse transcription PCR reference genes for gene expression analysis in teak (Tectona grandis L.f.)

Table 3 Descriptive statistics and expression level obtained by BestKeeper

Factor	Tgrp60s	TgSand	TgAct	TgCac	TgGAPDH	TgΒ-Tub	TgHis3	TgUbq	TgEf-1α
N	36	36	36	36	36	36	36	36	36
GM [CP]	28.59	29.92	26.21	31.05	25.63	29.59	28.24	26.77	23.45
AM [CP]	28.59	29.94	26.27	31.07	25.64	29.62	28.27	26.78	23.47
Min [CP]	27.52	28.08	24.15	28.71	24.21	28.00	26.31	25.99	22.50
Max [CP]	29.82	32.15	29.91	33.11	27.36	33.54	31.66	28.71	25.23
SD [±CP]	0.51	0.90	1.42	0.99	0.64	1.21	1.02	0.60	0.73
CV [%CP]	1.78	3.01	5.40	3.20	2.49	4.10	3.62	2.23	3.12
Min [x-fold]	-1.99	-3.82	-3.84	-4.58	-2.72	-2.62	-3.53	-1.63	-1.79
Max [x-fold]	2.22	5.13	11.12	3.85	3.38	10.89	9.29	3.37	2.99
SD [±x-fold]	1.39	1.79	2.50	1.90	1.51	2.19	1.94	1.47	1.61

Abbreviations: N: number of samples; CP: crossing point; GM [CP]: geometric CP mean; AM [CP]:arithmetic CP mean; Min [CP] and Max [CP]: CP threshold values; SD [±CP]: CP standard deviation; CV [%CP]: variance coefficient expressed as percentage of CP level; Min [x-fold] and Max [x-fold]: threshold expression levels expressed as absolute x-fold over- or under-regulation coefficient; SD [±x-fold]: standard deviation of absolute regulation coefficient.
SD and CV are indicated in bold.

ISSN: 1756-0500