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Table 1 Quantification of extracted DNA from fecal samples

From: Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction

Sample nr. NanoDrop® (μg/mL) Qubit® (μg/mL)
Qiagen EasyMag 35 μL silica EasyMag 140 μL silica Qiagen EasyMag 35 μL silica EasyMag 140 μL silica
HC-1 17.5 41.9 13.7 2.9 6.5 0.5
HC-2 40.1 9.6 41.4 6.9 0.7 1.2
HC-3 42.5 52.4 50.8 4.5 1.2 0.7
HC-8 18.5 10.3 36.1 3.4 1.3 0.6
HC-10 73.8 17.4 33.4 28.8 2.5 1.9
IBD-1* 9.5 6.7 32.2 0.6 0.8 1.0
IBD-2* 5.6 3.4 18.4 0.2 1.2 0.3
IBD-3* 55.5 5.9 21.2 2.7 0.4 0.5
Average 32.9 18.5 30.9 6.3 1.8 0.8
Standard deviation 24.1 18.4 12.5 9.4 2.0 0.5
  1. *Inflammatory Bowel Disease (IBD), Healthy controls (HC).
  2. The table shows faecal DNA concentration of extracts quantified using NanoDrop® and Qubit®. DNA extraction by EasyMag® with 35 μL and 140 μl silica yields in average 18.5 (SD 18.4) and 30.9 μg (SD 12.5) DNA, respectively and DNA extraction by Qiagen yields in average 32.9 μg (SD 24.1) DNA, using NanoDrop® instrument. Same faecal DNA extracts measured using Qubit® instrument for DNA by EasyMag® with 35 μL and 140 μL silica, yields a concentration of 1.8 (SD 2.0) and 0.8 μg (SD 0.5) DNA, respectively, while DNA extraction by Qiagen yields a concentration of 6.3 μg/ml (SD 9.4) DNA.