Table 1 Coverage results are shown for paired end mapping
From: Illumina sequencing of 15 deafness genes using fragmented amplicons
| Â | Patient 1 | Patient 2 | Patient 3 | Patient 4 |
|---|---|---|---|---|
Number of raw Illumina GAIIx reads | 12.81E + 06 | 8.56E + 06 | 3.15E + 06 | 3.03E + 06 |
Number of trimmed, quality filtered reads | 11.92E + 06 | 7.88E + 06 | 2.88E + 06 | 2.79E + 06 |
Mapped reads - Single end | 98.77% | 98.05% | 98.82% | 99.29% |
Mapped reads - Paired end | 99.02% | 98.35% | 99.14% | 99.57% |
(properly paired) | (97.71%) | (95.97%) | (96.82%) | (98.44%) |
Completely covered amplicons | 557/563 | 554/563 | 560/563 | 560/563 |
Average depth | 13064 | 8661 | 3329 | 3270 |
% of amplicon bases with depth > 0 | 99.42% | 99.30% | 99.36% | 99.55% |
% of amplicons bases with depth > 5 | 98.68% | 97.94% | 99.17% | 99.35% |