ICE Sde 3396 is transferable from SDSE, GAS and GBS. Arrows represent direction of transfer of the ICE into recipient strains. All three species are capable of acting as donors and recipients of the ICE. Transconjugants were initially identified on the basis of double antibiotic resistance phenotype. Transfer of the full ICE was confirmed by PCR amplification of DNA from Regions 1, 2 and 3 (data not shown).