Table 1 Primer sequences, repeat motifs, amplification parameters and accession numbers of microsatellite loci in Garra barreimiae
Locus name | Primer sequences 5′ - > 3′ | Primer labelling | Primer C (pMol) | Repeat motif | Reaction | Ta(°C) | Accession Nr. |
|---|---|---|---|---|---|---|---|
QLIM | F-ACGGTTGATAGAGCCACTGAC | 2 | AG | MPR1 | 65/60 | KM099076 | |
R-ACTGTCGAGCAATGCAACAC | PET | 2 | |||||
7GHL | F-GTATTACCGTGGTGATGTGGC | PET | 4 | AGAT | MPR1 | 65/60 | KM099077 |
R-GCGGCGTAGAAATCTGACTG | 4 | ||||||
PH8A | F-ACACTTCCAAAACGGTCGC | VIC | 2 | AGAT | MPR1 | 65/60 | KM099078 |
R-CGGAGCAAACCCAGTGACTA | 2 | ||||||
UHPE | F-ACCACAGCCAGTCAGATATGC | 2 | AC | MPR1 | 65/60 | KM099079 | |
R-GCTCAACAAGCTGAACTCCG | 6-FAM | 2 | |||||
JQSO | F-TTGGCTGGAGCGATGGCTG | 6-FAM | 2 | AC | MPR1 | 65/60 | KM099080 |
R-AGGGCTACATCACAGACTCAC | 2 | ||||||
DL6X | F-GTGACACATTTGGATAATGAATCAGC | NED | 2 | AC | TTR1 | 65/60 | KM099081 |
R-AGCTGGCCTAGATACCTGAC | 2 | ||||||
FYP9 | F-CGTCGACCAACGGACTAACG | NED | 2 | AC | TTR1 | 65/60 | KM099082 |
R-GACCGACATCAATCACGCGCT | 2 | ||||||
HOLN | F-ACTGCGCTCGTACCCTATG | 6-FAM | 2 | AC | TTR1 | 65/60 | KM099083 |
R-CAGCAGCCGGTAAATAGCTG | 2 | ||||||
JMLR | F-CGATCAGATATGCAATGGCAG | 2 | AC | MPR2 | 64/59 | KM099084 | |
R-GTGATCAGGCCTGTGTAAACG | NED | 2 | |||||
CJHG | F-CTGACCTGATCTACGAAACCG | 6-FAM | 1.6 | AC | MPR2 | 64/59 | KM099085 |
R-TGAGTTTGTGGAGTCCAGCG | 1.6 | ||||||
3Z71 | F-CGAGGTTTGGTTCCTCTTCTC | VIC | 1.6 | AG | MPR2 | 64/59 | KM099086 |
R-CAGCCTGGCTAACTCTGAGC | 1.6 | ||||||
9XNC | F-GCCAGTCAGTAGCTGCAAC | PET | 1.6 | AC | TTR2 | 64/59 | KM099087 |
R-GTTGTGCTATTCTGTGTAAGACC | 1.6 | ||||||
3 N43 | F-GTGCTGCTTTCCTTCTTCG | PET | 2 | AC | TTR2 | 64/59 | KM099088 |
R-ACCAGGAGCCGATCCATAAAC | 2 | ||||||
1EHE | F-GCAGATGTAGTCAATTGACATGC | 6-FAM | 1 | AC | TTR2 | 64/59 | KM099089 |
R-GCGAGGGCTTGAACTGATAC | 1 | ||||||
88CM | F-CTGTCGTCCATGGCTGTACC | VIC | 1 | AG | TTR2 | 64/59 | KM099090 |
R-AGGACTGGCATGTTTGCATT | 1 | ||||||
I6G2 | F-AGCATCTGAGCATTCTCCTG | VIC | 3 | AT | MPR3 | 60/54 | KM099091 |
R-CCAAACGGTACGTCTTATGG | 3 | ||||||
3ROZ | F-TGGTGGTTTATATGCAGTCTGA | VIC | 4 | AC | MPR3 | 60/54 | KM099092 |
R-ACCCAAGGATGTTGTGTCCG | 4 | ||||||
CB75 | F-CCATGCATCTACCTACCCAC | PET | 2 | AGGT | MPR3 | 60/54 | KM099093 |
R-TGATCAAATAGATGGTGGCTG | 2 | ||||||
2PUM | F-CATGCCAATTCACTCATTCC | NED | 1 | AG | TTR3 | 60/54 | KM099094 |
R-ACGTCACGTGTTGTCTATCTTG | 1 |