Skip to main content

Advertisement

Table 1 Primer sequences, repeat motifs, amplification parameters and accession numbers of microsatellite loci in Garra barreimiae

From: Characterization of 19 new microsatellite loci for the Omani barb Garra barreimiae from 454 sequences

Locus name Primer sequences 5′ - > 3′ Primer labelling Primer C (pMol) Repeat motif Reaction Ta(°C) Accession Nr.
QLIM F-ACGGTTGATAGAGCCACTGAC   2 AG MPR1 65/60 KM099076
  R-ACTGTCGAGCAATGCAACAC PET 2     
7GHL F-GTATTACCGTGGTGATGTGGC PET 4 AGAT MPR1 65/60 KM099077
  R-GCGGCGTAGAAATCTGACTG   4     
PH8A F-ACACTTCCAAAACGGTCGC VIC 2 AGAT MPR1 65/60 KM099078
  R-CGGAGCAAACCCAGTGACTA   2     
UHPE F-ACCACAGCCAGTCAGATATGC   2 AC MPR1 65/60 KM099079
  R-GCTCAACAAGCTGAACTCCG 6-FAM 2     
JQSO F-TTGGCTGGAGCGATGGCTG 6-FAM 2 AC MPR1 65/60 KM099080
  R-AGGGCTACATCACAGACTCAC   2     
DL6X F-GTGACACATTTGGATAATGAATCAGC NED 2 AC TTR1 65/60 KM099081
  R-AGCTGGCCTAGATACCTGAC   2     
FYP9 F-CGTCGACCAACGGACTAACG NED 2 AC TTR1 65/60 KM099082
  R-GACCGACATCAATCACGCGCT   2     
HOLN F-ACTGCGCTCGTACCCTATG 6-FAM 2 AC TTR1 65/60 KM099083
  R-CAGCAGCCGGTAAATAGCTG   2     
JMLR F-CGATCAGATATGCAATGGCAG   2 AC MPR2 64/59 KM099084
  R-GTGATCAGGCCTGTGTAAACG NED 2     
CJHG F-CTGACCTGATCTACGAAACCG 6-FAM 1.6 AC MPR2 64/59 KM099085
  R-TGAGTTTGTGGAGTCCAGCG   1.6     
3Z71 F-CGAGGTTTGGTTCCTCTTCTC VIC 1.6 AG MPR2 64/59 KM099086
  R-CAGCCTGGCTAACTCTGAGC   1.6     
9XNC F-GCCAGTCAGTAGCTGCAAC PET 1.6 AC TTR2 64/59 KM099087
  R-GTTGTGCTATTCTGTGTAAGACC   1.6     
3 N43 F-GTGCTGCTTTCCTTCTTCG PET 2 AC TTR2 64/59 KM099088
  R-ACCAGGAGCCGATCCATAAAC   2     
1EHE F-GCAGATGTAGTCAATTGACATGC 6-FAM 1 AC TTR2 64/59 KM099089
  R-GCGAGGGCTTGAACTGATAC   1     
88CM F-CTGTCGTCCATGGCTGTACC VIC 1 AG TTR2 64/59 KM099090
  R-AGGACTGGCATGTTTGCATT   1     
I6G2 F-AGCATCTGAGCATTCTCCTG VIC 3 AT MPR3 60/54 KM099091
  R-CCAAACGGTACGTCTTATGG   3     
3ROZ F-TGGTGGTTTATATGCAGTCTGA VIC 4 AC MPR3 60/54 KM099092
  R-ACCCAAGGATGTTGTGTCCG   4     
CB75 F-CCATGCATCTACCTACCCAC PET 2 AGGT MPR3 60/54 KM099093
  R-TGATCAAATAGATGGTGGCTG   2     
2PUM F-CATGCCAATTCACTCATTCC NED 1 AG TTR3 60/54 KM099094
  R-ACGTCACGTGTTGTCTATCTTG   1     
  1. Primer C (pMol) primer concentration in pMol, Ta (°C) annealing temperature, Primer labelling: DS-33 Dye set; accession numbers.