Table 1 Properties of the identified enzymes useful in RRBS
Enzyme | Consensus sequence | Restriction temp. (°C) | Digestion buffer | Heat inactivation (°C) | Methylation sensitive | Catalog number |
|---|---|---|---|---|---|---|
AluI | AG|CT | 37 | CutSmart | 80 | No | R0137S |
BfaI | C|TAG | 37 | CutSmart | 80 | No | R0568S |
HaeIII | GG|CC | 37 | CutSmart | 80 | No | R0108S |
HpyCH4V | TG|CA | 37 | CutSmart | 65 | No | R0620S |
MluCI | |AATT | 37 | CutSmart | No | No | R0538S |
MseI | T|TAA | 37 | CutSmart | 65 | No | R0525S |
MspI | C|CGG | 37 | CutSmart | No | No | R0106S |
TaqI | T|CGA | 65 | CutSmart | 80 | dam | R0149S |
CviQI | G|TAC | 25 | NEB 3.1 | No | No | R0639S |
CviAII | C|ATG | 25 | CutSmart | 65 | No | R0640S |
ApeKI | G|CWGC | 75 | NEB 3.1 | No | Yes | R0643S |