Adipose-derived stem cells from C57BL/6 mice observed under inverted microscopy after culture. For this study, were utilized 10 animals for sex, and experimental period of 30, 90, and 120 days (total of animals = 60). A and B: Initial explant of ADSCs (magnification, ×40 and × 20). It was observed in the cultures of adipose tissue, cells fusiform with appearance of fibroblast-like (arrowhead) and small cells adipose refractive rounded (arrow). C and D: Culture of ADSCs after 7 days (magnification, ×10 and × 20). E and F: Culture of ADSCs after 10 days. It is possible to observe cell confluence and homogenous population (magnification, ×5 and × 40). G and H: Culture of ADSCs above three passages on tissue culture plastic (magnification, ×20). Cell confluence was observed and homogenous population with small and lager cells in this period.