Analysis of SSEA-1 expression of ADSCs from C57BL/6 J mice males and females. Results represent means ± S.D. from three independent experiments of each experimental group (days and sexes) in triplicate, obtained by flow cytometry analysis. A: The graph shows ADSCs from mice males and females between 30 and 120 days of age characterized by flow cytometry compared with SSEA-1 expression. B, C, D, and E: A single fluorescent dye was used for a dot plot vs. FSC to visualize the expression of the markers of SSEA-1. B: SSEA-1 expression at female 30 days; C: SSEA-1 expression at female 120 days; D: SSEA-1 expression at male 30 days; E: SSEA-1 expression of male 120 days. Statistical differences were obtained by analysis of variance ANOVA and Tukey-Kramer multiple-comparisons test. Statistical significance (p-value) *p < 0.05 and **p < 0.01.