Analysis of cyclin D1 expression of ADSCs from C57BL/6 J mice males and females . Results represent means ± S.D. from three independent experiments of each experimental group (days and sexes) in triplicate, obtained by flow cytometry analysis. The graph shows ADSCs from mice males and females among 30, 90, and 120 days of age subdivided into morphologically large and small cells after characterization by flow cytometry compared with cyclin D1 expression. Statistical differences were obtained by analysis of variance ANOVA and Tukey-Kramer multiple-comparisons test. No significant (ns) difference at the groups.