Figure 5

Effect of pH and temperature on the oxalate-degrading activity. Purified OXDC was incubated with oxalate at pH 5, 6 or 7 and enzyme activity of purified OXDC was determined by oxalate kit to investigate the optimal pH and temperature. Enzyme activity of purified OXDC (pH 5, pH 6 or pH 7) was measured at 28°C (A) or 37°C (B). To investigate the optimal temperature, enzyme activity of purified OXDC at pH 5 was determined at 10°C, 20°C, 28°C, 37°C, 42°C, 60°C, or 70°C (C).