Isolation and characterization of 11 novel microsatellite loci in a West African leaf-nosed bat, Hipposideros aff. ruber

Heather J Baldwin; Peter Vallo; Michael G Gardner; Christian Drosten; Marco Tschapka; Adam J Stow

doi:10.1186/1756-0500-7-607

BMC Research Notes

Table 1 Characteristics and thermocycling conditions for 11 polymorphic microsatellites in the African leaf-nosed bat Hipposideros aff. ruber

From: Isolation and characterization of 11 novel microsatellite loci in a West African leaf-nosed bat, Hipposideros aff. ruber

Locus	Accession #	Repeat motif	Primer sequences (5′ – 3′)	MgCl₂/P_FR	T a (°C)	Size (bp)	N	N_A	H_O	H_E	HWE	P_NULL
Hr1	KM370156	(GATA)₁₃	F:TGGCAAGGTTAACACGAACC	2.0 mM/0.5 μM	60-50	238-258	38	6	0.74	0.79	ns	0.028
Hr1	KM370156	(GATA)₁₃	R:TCTCCCTCCCGCTCTTATCT	2.0 mM/0.5 μM	60-50	238-258	38	6	0.74	0.79	ns	0.028
Hr2	KM370157	(TCTT)₁₅	F:GAAGCACTGCTGGAAAGGTT	2.0 mM/0.25 μM	60-50	311-339	34	8	0.77	0.76	ns	-0.009
Hr2	KM370157	(TCTT)₁₅	R:GTTGAACTGGGTGGCCTTTA	2.0 mM/0.25 μM	60-50	311-339	34	8	0.77	0.76	ns	-0.009
Hr5	KM370160	(GAAG)₁₄	F:TGGGTGTTTCAGTTTCATGC	2.0 mM/0.5 μM	65-55	186-234	34	9	0.82	0.82	ns	-0.006
Hr5	KM370160	(GAAG)₁₄	R:TGGTCTATTTGTTTCCTTCCGTA	2.0 mM/0.5 μM	65-55	186-234	34	9	0.82	0.82	ns	-0.006
Hr6	KM370161	(TCTT)₁₃	F:GGGTTTCTTCAAATGTGTTTTC	2.0 mM/0.5 μM	55-47	204-240	37	8	0.70	0.73	ns	0.012
Hr6	KM370161	(TCTT)₁₃	R:GCCTCCAAGACAAACAGAGG	2.0 mM/0.5 μM	55-47	204-240	37	8	0.70	0.73	ns	0.012
Hr7	KM370162	(ATTT)₁₁	F:AGCCAATGACAAGACTGCCTA	2.0 mM/0.5 μM	65-55	144-172	33	8	0.42	0.68	***	0.173
Hr7	KM370162	(ATTT)₁₁	R:CCAGTGAAGCAACGTCCTCT	2.0 mM/0.5 μM	65-55	144-172	33	8	0.42	0.68	***	0.173
Hr8	KM370163	(ATCT)₁₂	F:CTCAGCCCAAAGTCAAGGAG	2.0 mM/0.5 μM	60-50	221-241	36	6	0.72	0.68	ns	-0.042
Hr8	KM370163	(ATCT)₁₂	R:TGGCTATACGAATACAAAGATTAGACA	2.0 mM/0.5 μM	60-50	221-241	36	6	0.72	0.68	ns	-0.042
Hr9	KM370164	(TCTA)₁₂	F:TGCTATCTTCCATGAGGTCAGA	2.0 mM/0.5 μM	60-50	218-234	38	5	0.63	0.73	ns	0.061
Hr9	KM370164	(TCTA)₁₂	R:TCTCTGTTGCTGAAGGAAAACTT	2.0 mM/0.5 μM	60-50	218-234	38	5	0.63	0.73	ns	0.061
Hr10	KM370165	(TTAT)₁₁	F:TCCACTGGAGTAAGAGATGTGTG	2.0 mM/1.0 μM	65-55	258-282	38	7	0.79	0.74	ns	-0.040
Hr10	KM370165	(TTAT)₁₁	R:GCACTGCAACAGTGAAAAGC	2.0 mM/1.0 μM	65-55	258-282	38	7	0.79	0.74	ns	-0.040
Hr11	KM370166	(TTTC)₁₄	F:CTCTTGCAATGAAGGCAATG	2.0 mM/0.5 μM	65-55	106-154	37	12	0.87	0.86	ns	-0.018
Hr11	KM370166	(TTTC)₁₄	R:CTGCCATGAGCTACCATGAG	2.0 mM/0.5 μM	65-55	106-154	37	12	0.87	0.86	ns	-0.018
Hr12	KM370167	(GATA)₁₂	F:TTGGTTTTCAGATCTTCTGGTG	2.5 mM/0.5 μM	60-50	277-293	38	4	0.42	0.60	**	0.140
Hr12	KM370167	(GATA)₁₂	R:GAGTCTTCTGCCTGCTGGAC	2.5 mM/0.5 μM	60-50	277-293	38	4	0.42	0.60	**	0.140
Hr13	KM370168	(TTTC)₁₃	F:CCGAAGCCAATCTGGTTTTA	2.0 mM/1.0 μM	65-55	321-329	34	2	0.00	0.06	ns	0.157
			R:GGGTCCTGCAGAAACACACT

P_FR forward and reverse primer concentration, T a annealing temperatures of touchdown cycles (see Methods), N number of individuals, N_A number of alleles, H_O observed heterozygosity, H_E expected heterozygosity, HWE probability of deviation from Hardy-Weinberg equilibrium, P_NULL null allele frequency estimate (van Oosterhout), ns not significant., **p < 0.01, ***p < 0.001.

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