Locus
|
Accession #
|
Repeat motif
|
Primer sequences (5′ – 3′)
|
MgCl2/PFR
|
T a (°C)
|
Size (bp)
|
N
|
NA
|
HO
|
HE
|
HWE
|
PNULL
|
---|
Hr1
|
KM370156
|
(GATA)13
|
F:TGGCAAGGTTAACACGAACC
|
2.0 mM/0.5 μM
|
60-50
|
238-258
|
38
|
6
|
0.74
|
0.79
|
ns
|
0.028
|
R:TCTCCCTCCCGCTCTTATCT
|
Hr2
|
KM370157
|
(TCTT)15
|
F:GAAGCACTGCTGGAAAGGTT
|
2.0 mM/0.25 μM
|
60-50
|
311-339
|
34
|
8
|
0.77
|
0.76
|
ns
|
-0.009
|
R:GTTGAACTGGGTGGCCTTTA
|
Hr5
|
KM370160
|
(GAAG)14
|
F:TGGGTGTTTCAGTTTCATGC
|
2.0 mM/0.5 μM
|
65-55
|
186-234
|
34
|
9
|
0.82
|
0.82
|
ns
|
-0.006
|
R:TGGTCTATTTGTTTCCTTCCGTA
|
Hr6
|
KM370161
|
(TCTT)13
|
F:GGGTTTCTTCAAATGTGTTTTC
|
2.0 mM/0.5 μM
|
55-47
|
204-240
|
37
|
8
|
0.70
|
0.73
|
ns
|
0.012
|
R:GCCTCCAAGACAAACAGAGG
|
Hr7
|
KM370162
|
(ATTT)11
|
F:AGCCAATGACAAGACTGCCTA
|
2.0 mM/0.5 μM
|
65-55
|
144-172
|
33
|
8
|
0.42
|
0.68
|
***
|
0.173
|
R:CCAGTGAAGCAACGTCCTCT
|
Hr8
|
KM370163
|
(ATCT)12
|
F:CTCAGCCCAAAGTCAAGGAG
|
2.0 mM/0.5 μM
|
60-50
|
221-241
|
36
|
6
|
0.72
|
0.68
|
ns
|
-0.042
|
R:TGGCTATACGAATACAAAGATTAGACA
|
Hr9
|
KM370164
|
(TCTA)12
|
F:TGCTATCTTCCATGAGGTCAGA
|
2.0 mM/0.5 μM
|
60-50
|
218-234
|
38
|
5
|
0.63
|
0.73
|
ns
|
0.061
|
R:TCTCTGTTGCTGAAGGAAAACTT
|
Hr10
|
KM370165
|
(TTAT)11
|
F:TCCACTGGAGTAAGAGATGTGTG
|
2.0 mM/1.0 μM
|
65-55
|
258-282
|
38
|
7
|
0.79
|
0.74
|
ns
|
-0.040
|
R:GCACTGCAACAGTGAAAAGC
|
Hr11
|
KM370166
|
(TTTC)14
|
F:CTCTTGCAATGAAGGCAATG
|
2.0 mM/0.5 μM
|
65-55
|
106-154
|
37
|
12
|
0.87
|
0.86
|
ns
|
-0.018
|
R:CTGCCATGAGCTACCATGAG
|
Hr12
|
KM370167
|
(GATA)12
|
F:TTGGTTTTCAGATCTTCTGGTG
|
2.5 mM/0.5 μM
|
60-50
|
277-293
|
38
|
4
|
0.42
|
0.60
|
**
|
0.140
|
R:GAGTCTTCTGCCTGCTGGAC
|
Hr13
|
KM370168
|
(TTTC)13
|
F:CCGAAGCCAATCTGGTTTTA
|
2.0 mM/1.0 μM
|
65-55
|
321-329
|
34
|
2
|
0.00
|
0.06
|
ns
|
0.157
|
| | |
R:GGGTCCTGCAGAAACACACT
| | | | | | | | | |
- PFR forward and reverse primer concentration, T a annealing temperatures of touchdown cycles (see Methods), N number of individuals, NA number of alleles, HO observed heterozygosity, HE expected heterozygosity, HWE probability of deviation from Hardy-Weinberg equilibrium, PNULL null allele frequency estimate (van Oosterhout), ns not significant., **p < 0.01, ***p < 0.001.