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Table 1 A comparison between standard and modified H&E staining protocol durations

From: Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection

Standard H&E staining Modified H&E staining
Steps Reagent Time Steps Reagent Time
1 Haematoxylin 2 2 mins 1 Air dry slides in cryostat at −16°C 2 mins
2 Gentle running water 2 mins 2 70% Ethanol 30 secs
3 Clarifier 2 2 mins 3 Water 5 secs
4 Gentle running water 2 mins 4 Hematoxylin 2 10 secs
5 Bluing solution 2 mins 5 Bluing reagent 10 secs
6 Gentle running water 2 mins 6 70% Ethanol 10 secs
7 70% Ethanol 2 mins 7 Eosin/Phloxine 2 secs
8 Eosin/Phloxine 15 secs 8 100% Ethanol 5 secs
9 70% Ethanol 2 mins 9 100% Ethanol 30 secs
10 95% Ethanol 2 mins 10 100% Ethanol 30 secs
11 100% Ethanol 2 mins 11 Xylene 5 secs
12 100% Ethanol 2 mins 12 Xylene 30 secs
13 100% Ethanol 2 mins 13 Xylene 1 min
14 Xylene 2 mins Total time ~5 mins
Total time ~26 mins