Standard H&E staining | Modified H&E staining | ||||
---|---|---|---|---|---|
Steps | Reagent | Time | Steps | Reagent | Time |
1 | Haematoxylin 2 | 2 mins | 1 | Air dry slides in cryostat at −16°C | 2 mins |
2 | Gentle running water | 2 mins | 2 | 70% Ethanol | 30 secs |
3 | Clarifier 2 | 2 mins | 3 | Water | 5 secs |
4 | Gentle running water | 2 mins | 4 | Hematoxylin 2 | 10 secs |
5 | Bluing solution | 2 mins | 5 | Bluing reagent | 10 secs |
6 | Gentle running water | 2 mins | 6 | 70% Ethanol | 10 secs |
7 | 70% Ethanol | 2 mins | 7 | Eosin/Phloxine | 2 secs |
8 | Eosin/Phloxine | 15 secs | 8 | 100% Ethanol | 5 secs |
9 | 70% Ethanol | 2 mins | 9 | 100% Ethanol | 30 secs |
10 | 95% Ethanol | 2 mins | 10 | 100% Ethanol | 30 secs |
11 | 100% Ethanol | 2 mins | 11 | Xylene | 5 secs |
12 | 100% Ethanol | 2 mins | 12 | Xylene | 30 secs |
13 | 100% Ethanol | 2 mins | 13 | Xylene | 1Â min |
14 | Xylene | 2 mins | Total time | ~5 mins | |
Total time | ~26 mins | Â | Â | Â |