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Table 1 A comparison between standard and modified H&E staining protocol durations

From: Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection

Standard H&E staining

Modified H&E staining

Steps

Reagent

Time

Steps

Reagent

Time

1

Haematoxylin 2

2 mins

1

Air dry slides in cryostat at −16°C

2 mins

2

Gentle running water

2 mins

2

70% Ethanol

30 secs

3

Clarifier 2

2 mins

3

Water

5 secs

4

Gentle running water

2 mins

4

Hematoxylin 2

10 secs

5

Bluing solution

2 mins

5

Bluing reagent

10 secs

6

Gentle running water

2 mins

6

70% Ethanol

10 secs

7

70% Ethanol

2 mins

7

Eosin/Phloxine

2 secs

8

Eosin/Phloxine

15 secs

8

100% Ethanol

5 secs

9

70% Ethanol

2 mins

9

100% Ethanol

30 secs

10

95% Ethanol

2 mins

10

100% Ethanol

30 secs

11

100% Ethanol

2 mins

11

Xylene

5 secs

12

100% Ethanol

2 mins

12

Xylene

30 secs

13

100% Ethanol

2 mins

13

Xylene

1 min

14

Xylene

2 mins

Total time

~5 mins

Total time

~26 mins

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