Table 2 RIN of tissue sections or PTCs at progressive stages of sample preparation
Experimental conditions | RIN |
|---|---|
Fresh tissue section without OCT embedding and H&E stain | 8 |
Fresh tissue section with OCT embedding but without H&E stain | 8.1 |
Fresh tissue scrape with OCT embedding and H&E stain | 7 |
Tissue sections stored in −80°C for a day | 7.5 |
Tissue sections stored in −80°C for a week | 6.5 |
Tissue sections stored in −80°C for 1 month | 6 |
Tissue sections stored in −80°C for 6 months | 2.2 |
PTCs collected within 15 minutes after H&E staining | 6.2 |
PTCs collected within 35 minutes after H&E staining | 1 |