Figure 2From: Rapid establishment of a HEK 293 cell line expressing FVIII-BDD using AAV site-specific integration plasmidsGFP expression of a selected stable cell clone of pTRP5GFPFVIII-BDD transduced HEK 293 cell. A). Green fluorescent microscope picture of the pTRP5GFPFVIII-BDD transduced stable HEK 293 cell line. Scale bar corresponds to 10 μm. B). Flow cytometric analysis of GFP expression of a selected stable cell clone of pTRP5GFPFVIII-BDD transduced HEK 293 cell. (a) Negative HEK 293 cell; (b) The cloned HEK 293 cell line, showing that 99.9% were GFP positive.Back to article page