Figure 4From: Rapid establishment of a HEK 293 cell line expressing FVIII-BDD using AAV site-specific integration plasmidsFVIII-BDD protein expression in the cloned cell lines. The total proteins in the cultured serum-free media of cloned cells were concentrated to identify the expression of FVIII-BDD proteins, and probed with Factor VIII (S2194) pAb. Lane 1. HEK 293-FVIII-BDD cells; Lane 2. Mock HEK 293 cells.Back to article page