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Figure 1 | BMC Research Notes

Figure 1

From: Long-term storage of blood RNA collected in RNA stabilizing Tempus tubes in a large biobank – evaluation of RNA quality and stability

Figure 1

The effect of long-term storage on RNA yield. Adult (n = 75 Tempus tubes from 3 donors) and cord (n = 30 Tempus tubes from 3 donors) blood samples were collected in Tempus tubes (3 ml blood per tube) and stored at -80°C for up to six years, followed by total RNA extraction. Each Tempus tube was considered as an independent biological sample. A) The RNA yield from the adult blood samples (n = 15 Tempus tubes/year). B) The RNA yield from cord blood samples (n = 6 Tempus tubes/year). Significantly lower RNA yields were recorded for cord blood samples analyzed in year 2 and 4. The average total RNA yields for adult and cord blood samples were 7.3 ± 2.6 μg per Tempus tube and 65.5 ± 20.4 μg per Tempus tube, respectively. The RNA yields in cord blood samples were significantly higher than in the adult blood samples. Each bar represents the average RNA yield and the error bar indicates ± SE.

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