Figure 4From: Long-term storage of blood RNA collected in RNA stabilizing Tempus tubes in a large biobank – evaluation of RNA quality and stabilityLong-term storage effects on RNA transcript levels. The transcript levels for six genes were analyzed by RT-qPCR, using RNA extracted from adult blood and cord blood samples collected in Tempus tubes stored at –80°C until analysis. A total of 21 Tempus Blood RNA tubes (15 adult blood samples and 6 cord blood samples) were analyzed in each year. A) Relative transcript levels of six genes from adult blood samples (n = 15 Tempus tubes/year). B) Relative transcript levels of six genes from cord blood samples (n = 6 Tempus tubes/year). The year 0 samples were used as reference (calibrators) and all other samples were compared against the reference samples. The dashed lines indicate ± 2-fold. Each bar represents the average log2-transformed fold change values and the error bar indicates ± SE.Back to article page