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Figure 5 | BMC Research Notes

Figure 5

From: Long-term storage of blood RNA collected in RNA stabilizing Tempus tubes in a large biobank – evaluation of RNA quality and stability

Figure 5

Variations in average Cq-values within and between donors. The coefficients of variation (CV) of the average Cq-values were calculated for adult and cord blood samples collected in Tempus tubes and stored at –80°C until analysis for the expression of six genes. The CVs within a donor (intra-donor) and between donors (inter-donor) across the storage years are presented. A) Adult blood samples. CVs ranged from 0.45% to 3.84%. B) Cord blood samples. CVs ranged from 0.12% to 4.74%. CV of 5% is indicated by a stippled line; CVs for all samples were considerably lower reflecting minor variation in the average Cq-values. Each point represents the average CV of samples from one donor.

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BMC Research Notes

ISSN: 1756-0500

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