Chloroplast localization and dimerization of FLAG-tagged HSP90.5. A, Immunoblotting of total plant leaf lysates and isolated chloroplast lysates from normally growing transgenic lines no. 3 (3G), 4, 7 and variegated no. 8 (8A) lines as well as from wild type (WT) plants using anti-FLAG and anti-HSP90.2 antibodies. SDS-PAGE of 10 μg proteins stained with Coomassie brilliant blue is shown to indicate equal loading. B, Size exclusion chromatography of purified mature forms of HSP90.5 and HSP90.5FLAG. The fractions were separated by 12% SDS-PAGE and stained with Coomassie brilliant blue.