Figure 4

Analysis of GUS enzyme activity in stable GUS expressing ECS line. GUS enzyme activity of stable GUS expressing line from 2 to 540 days after transformation with vector pFAJ3000 (p35S::gusA^INT). From 2 to 35 days cell clusters were proliferating under selective conditions [ZZ medium (pH 5.8) supplemented with 50 mg L⁻¹ geneticin, 200 mg L⁻¹ timentin], while 540 days after transformation GUS enzyme activity was measured in leaf tissue of in vitro plants. Bars represent the mean ± standard deviation (SD). Each mean value was calculated from six independent biological replicates (n = 6) (one replicate of proliferating cell clusters consisted of multiple clusters, while leaf tissue of one plantlet was used per replicate).