Figure 7
From: Assessment of RNAi-induced silencing in banana (Musa spp.)
Relation between GUS and siRNA expression in transgenic in vitro plantlets containing the pIMHKUL3 vector. (A) GUS enzyme activity in leaves of 10 independent pIMHKUL3 transgenic lines (1–10) and of 2 pSTARGATE control lines (C1, C2) regenerated after transformation of a stable GUS expressing (pFAJ3000 transformed) ECS. Bars represented the mean ± SD. Mean values were calculated from two independent measurements per line. (B) Northern blot detection of small RNA of ~21 nt using a gusA RNA probe. RNA was isolated from a portion of the leaves used for the GUS enzyme activity assay in (A). (C) 25S rRNA from the Northern gel blot from (B) was used as loading control.