Figure 5
Phage immunoassay of the enrichment against target antigens. The enrichment of phages is shown in the three selection schemes: selection against streptavidin (Panels A, D and G), selection against digoxigenin with STR>STR>AVI-carrier strategy (Panels B, E and H) and selection against digoxigenin with STR>AVI>STR-carrier strategy (Panels C, F and I). Samples have been divided further according to superinfection (Panels A, B and C), fusion gene (Panels D, E and F) and antibody format (Panels G, H and I). Open symbols: first repeat and filled symbols: second repeat. 1 × 109 rcfu phages were applied on STR and BSA wells (streptavidin) or DIG and biotin wells (digoxigenin) and revealed by europium-labeled anti-phage antibody. The data is shown as the STR/BSA- and DIG/biotin-signal ratio (S/B) for each sample. All sample points measured as triplicate (CV < 39% at all measured points with a median at 19%). Error bars excluded for clarity.