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Table 1 Library size, quality and phage titer

From: The selection performance of an antibody library displayed on filamentous phage coat proteins p9, p3 and truncated p3

Phagemid

Fusion protein

Transformed

Superinfection

In-frame

Phage yieldaI, cfu

Phage yieldaII, cfu

pAK400-Fab

LC-BLA

2 × 107

None

8/8

-

-

pEB07-Fab

Fd-BLA

3 × 108

None

8/8

-

-

pEB30-Fab

Fd-p3

3 × 109

None

22/24

-

-

pEB30-Fab

Fd-p3

-“-

Hyperphage

7/23

1.8 ± 0.3 × 1012

2.9 ± 1.0 × 1011

pEB30-Fab

Fd-p3

-“-

VCS-M13

8/22

7.1 ± 0.5 × 1013

3.6 ± 0.8 × 1012

pEB32x-Fab

Fd-p3Δ

1 × 109

VCS-M13

22/24

1.5 ± 0.6 × 1014

1.1 ± 0.1 × 1014

pEB92-Fab

Fd-p9

5 × 109

VCS-M13

22/23

3.3 ± 0.2 × 1014

2.9 ± 0.8 × 1014

pEB32x-scFv

Fd-p3Δ

6 × 109

VCS-M13

21/34

3.2 ± 0.3 × 1014

3.1 ± 7.4 × 1014

  1. aPhage yield of the display stocks as infective titer per 500 ml cell culture. Titer was measured twice independently (I and II) of three sample dilution series for each sample. Uncertainty informed as the standard deviation of the replica dilutions.