Table 3 Sequenced anti-DIG clone diversity and highest observed affinities
Digoxigenin STR > STR > AVI | Digoxigenin STR > AVI > STR | ||||||
|---|---|---|---|---|---|---|---|
Library | Helper phage | N | D | Highest aff. (IC50) | N | D | Highest aff. (IC50) |
Fab-p3 | Hyperphage | 8 | 1 | 442 ± 37 nM | 8 | 4 | 442 ± 37 nM |
Fab-p3 | VCS-M13 | 8 | 1 | 442 ± 37 nM | 8 | 2 | 442 ± 37 nMa |
Fab-p3 | Hyper > VCS | 8 | 1 | 442 ± 37 nM | 8 | 1 | 442 ± 37 nM |
Fab-p3Δ | VCS-M13 | 7 | 4 | 442 ± 37 nM | 7b | 5 | 1.5 ± 0.1 μM |
ScFv-p3Δ | VCS-M13 | 8 | 4 | 158 ± 20 nM | 8 | 6 | 112 ± 24 nM |
Fab-p9 | VCS-M13 | 3 | 2 | 442 ± 37 nM | 0 | 0 | N.D. |