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Table 1 Spontaneous lymphocyte proliferation in the presence of IL-2 added either at the beginning or towards the end of culture

From: Proliferation assay amplification by IL-2 in model primary and recall antigen systems

  Lymphocyte proliferation (cpm)
Donor No IL-2 IL-2 (60 i.u./ml) added IL-2 (600 i.u./ml) added
   Day 0 Day 5 Day 0 Day 5
M1 67 10306 - 28138 206
F1 66 17645 221 46194 238
F2 127 2091 160 - -
F4 92 934 125 - -
F5 105 866 128 - -
  1. PBMC from one male and four female donors were cultured at 0.15 × 106 cells/ml for eight days. IL-2 at a final concentration of 60 or 600 i.u./ml was added either at the start of culture or after 5 days. Tritiated thymidine was present for the final 18 h of culture and mean values of thymidine uptake are shown.