Proliferation assay amplification by IL-2 in model primary and recall antigen systems

BMC Research Notes

Table 1 Spontaneous lymphocyte proliferation in the presence of IL-2 added either at the beginning or towards the end of culture

	Lymphocyte proliferation (cpm)
Donor	No IL-2	IL-2 (60 i.u./ml) added		IL-2 (600 i.u./ml) added
		Day 0	Day 5	Day 0	Day 5
M1	67	10306	-	28138	206
F1	66	17645	221	46194	238
F2	127	2091	160	-	-
F4	92	934	125	-	-
F5	105	866	128	-	-

PBMC from one male and four female donors were cultured at 0.15 × 10⁶ cells/ml for eight days. IL-2 at a final concentration of 60 or 600 i.u./ml was added either at the start of culture or after 5 days. Tritiated thymidine was present for the final 18 h of culture and mean values of thymidine uptake are shown.

ISSN: 1756-0500