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Table 2 IL-2 amplification of proliferation assay

From: Proliferation assay amplification by IL-2 in model primary and recall antigen systems

  Lymphocyte proliferation (cpm)
KLH (μg/ml) KLH (μg/ml) + IL-2 PPD PPD + IL-2
Donor No antigen 0.01 0.1 1 10 No antigen 0.01 0.1 1 10
M1 148 - 203 568 376 175 - 1981 2676 2004 6358c 17702
M1 72 70 90 104 - 163 437 1253 1070 - 1426b 8431
M1 108 - 113 128 279 221 - 429 1732 1178 419a 4846
F2 71 90 139 1241 - 123 212 380 2969 - 9233c 16818
F3 83 - 93 239 - 115 - 569 1163 - 216b 1691
F4 90 102 86 175 - 148 124 283 521 - 965b 5284
  1. Mean values of thymidine uptake are shown for one male and three female donors in a total of six experiments. PBMC were cultured at 0.15 × 106 cells/ml. IL-2 at 60 i.u./ml was added, where indicated, after 5 days. KLH or PPD were present from the start of culture as shown. PPD concentration in the different experiments is indicated by (a) 0.1, (b) 1 or (c) 10 μg/ml.