Proliferation assay amplification by IL-2 in model primary and recall antigen systems

BMC Research Notes

Table 2 IL-2 amplification of proliferation assay

	Lymphocyte proliferation (cpm)
	KLH (μg/ml)					KLH (μg/ml) + IL-2					PPD	PPD + IL-2
Donor	No antigen	0.01	0.1	1	10	No antigen	0.01	0.1	1	10	PPD	PPD + IL-2
M1	148	-	203	568	376	175	-	1981	2676	2004	6358^c	17702
M1	72	70	90	104	-	163	437	1253	1070	-	1426^b	8431
M1	108	-	113	128	279	221	-	429	1732	1178	419^a	4846
F2	71	90	139	1241	-	123	212	380	2969	-	9233^c	16818
F3	83	-	93	239	-	115	-	569	1163	-	216^b	1691
F4	90	102	86	175	-	148	124	283	521	-	965^b	5284

Mean values of thymidine uptake are shown for one male and three female donors in a total of six experiments. PBMC were cultured at 0.15 × 10⁶ cells/ml. IL-2 at 60 i.u./ml was added, where indicated, after 5 days. KLH or PPD were present from the start of culture as shown. PPD concentration in the different experiments is indicated by (a) 0.1, (b) 1 or (c) 10 μg/ml.

ISSN: 1756-0500