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Table 1 Comparison of cell growth during 4 h induction at 28°C (after addition of 0.55 mM IPTG at absorbance 0.75) of E. coli BL21 (DE3) Star™/pAE/LigB (131-645aa) after two pre-induction growth temperatures, 28°C and 37°C, at 200 rpm in TB medium with the addition of 0.4% glycerol, 1% de glucose and 100 μg/mL ampicillin

From: Evaluation of pre-induction temperature, cell growth at induction and IPTG concentration on the expression of a leptospiral protein in E. coli using shaking flasks and microbioreactor

  Cell growth (Abs600nm) Soluble LigB (131-645aa) expression (mg/L)
Post induction time (h) Tgrowth (pre-induction)28°C Tgrowth (pre-induction)37°C Tgrowth (pre-induction)28°C Tgrowth (pre-induction)37°C
Induction 0.75 0.76 - -
1 h 1.36 1.40 17 15
2 h 1.69 1.80 80 67
3 h 2.25 2.29 108 118
4 h 2.73 2.97 176 186