Figure 1

Biochemical changes detected in skeletal muscle, liver, and adipose tissue after 5 week treatment with either R118 or metformin. Male HFD DIO C57BL/6 mice (21 weeks old, 17 weeks on HFD) were fed using control HFD, HFD formulated with 200 mg/kg R118, or HFD formulated with 5 g/kg metformin (n = 12/group). After five weeks of treatment, liver, skeletal muscle, adipose tissue, and plasma were collected for half of the mice in each group. For the remaining mice, compound-formulated HFD was replaced with control HFD for 24 hours (washout), followed by similar sample collection. For each tissue, the number of metabolites where the difference between drug-treated versus control HFD has p < 0.05 is shown in the graphs. Further breakdown of the significant changes into metabolites that are increased versus decreased compared to the control HFD is indicated by the color coding, where green is decreased with drug treatment relative to HFD control and red is increased with drug treatment relative to control HFD.