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Figure 4 | BMC Research Notes

Figure 4

From: Global metabolite profiling of mice with high-fat diet-induced obesity chronically treated with AMPK activators R118 or metformin reveals tissue-selective alterations in metabolic pathways

Figure 4

Metformin has a more pronounced effect on hepatic glucose metabolism and long chain fatty acids compared to R118. Male HFD DIO C57BL/6 mice (21 weeks old, 17 weeks on HFD) were fed using control HFD, HFD formulated with 200 mg/kg R118, or HFD formulated with 5 g/kg metformin (n = 12/group). After five weeks of treatment, liver, skeletal muscle, adipose tissue, and plasma were collected for half of the mice in each group (max cmp). For the remaining mice, compound-formulated HFD was replaced with control HFD for 24 hours (washout), followed by similar sample collection. Detected changes in hepatic intermediates of glucose metabolism (Panel A) and long chain fatty acids (Panel B) for both R118 and metformin-treated mice are shown relative to control HFD in heatmap format. Biochemical data were analyzed using Welch’s two-sample t-tests.

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