Figure 3

Most stable to least stable gene expression analysis in embryos cultured in KSOM medium based on their expression stability: (A) 2-cell, (B) 4- cell, C) 8-cell, (D) morulae, and (E) blastocyst stages. Data were obtained from A–E. Ranking is based on the principle that gene pairs have stable expression patterns relative to each other and are considered appropriate reference genes. The most stable genes are on the left and the least stable genes on the right along the X-axis. The experiments were performed in triplicate; data shown represent the mean of three independent experiments.