Table 1 Primer sets used in this study
Gene name | Accession number | Primer sequences (forward/reverse) | Tm(°C) | Amplication length | Amplification efficacy (%) |
|---|---|---|---|---|---|
h2afz | NM_016750 | GTGGACTGTATCTCTGTGAA/GGTTGGTTGGAAGGCTAA | 60 | 89 | 92.1 |
sdha | NM_023281 | ATTCATTGTCTACTTCTCACT/AGGGTTTATTTGGCTTACA | 58 | 108 | 90.6 |
tgfb1 | NM_011577 | TATACTGAGACACCTTGG/GTGATAGTCCTGAATAATTTG | 55 | 83 | 97.2 |
gapdh | NM_008084 | AGTGGCAAAGTGGAGATT/GTGGAGTCATACTGGAACA | 60 | 83 | 91.5 |
actb | NM_007393 | ATCTTCCGCCTTAATACT/GCCTTCATACATCAAGTT | 56 | 98 | 90.7 |
sptbn | NM_175836 | TCTAATGGTTACTTGCTTGT/CAATAGTTACAGTGACAGAGA | 55 | 101 | 91.2 |
ablim | NM_178688 | GTATTCAGTGTTCACAGT/AATAGCATTAACCAGTAAGA | 55 | 106 | 90.1 |
ywhaz | NM_001253807 | CAGTAGATGGAGAAAGATTTGC/GGGACAATTAGGGAAGTAAGT | 60 | 92 | 93.5 |
wrnip | NM_030215 | ATGAGTAGGATGCTTGTA/TAACCACCTCCATCTATG | 56 | 130 | 91.6 |
18Â s | X00686 | CGCCGCTAGAGGTGAAATTCT/CGAACCTCCGACTTTCGTTCT | 60 | 102 | 93.6 |