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Table 3 Ranking of reference genes according to development stages of pre-implantation embryo stages

From: Evaluation of reference genes in mouse preimplantation embryos for gene expression studies using real-time quantitative RT-PCR (RT-qPCR)

Medium

Ranking

2C

4C

8C

Mo

Bl

KSOM

1

wrnip

wrnip

h2afz

ywhaz

ablim

CZB

h2afz

h2afz

actb

h2afz

wrnip

M16

h2afz

h2afz

ywhaz

h2afz

h2afz

in-vivo

tgfb1

18 s

18 s

ywhaz

h2afz

KSOM

2

tgfb1

h2afz

sptbn

ablim

actb

CZB

tgfb1

tgfb1

h2afz

ywhaz

h2afz

M16

sptbn

sptbn

sptbn

sptbn

tgfb1

in-vivo

ywhaz

sptbn

h2afz

h2afz

ywhaz

KSOM

3

ablim

ablim

tgfb1

h2afz

sptbn

CZB

wrnip

ywhaz

ywhaz

tgfb1

ywhaz

M16

ywhaz

sdha

sdha

sdha

sptbn

in-vivo

18 s

ywhaz

gapdh

18 s

18 s

KSOM

4

h2afz

sptbn

gapdh

sptbn

18 s

CZB

sdha

18 s

sdha

wrnip

actb

M16

sdha

ywhaz

h2afz

ywhaz

ywhaz

in-vivo

sptbn

gapdh

ywhaz

gapdh

actb

KSOM

5

18 s

tgfb1

ablim

wrnip

h2afz

CZB

18 s

sdha

18 s

actb

sptbn

M16

wrnip

tgfb1

18 s

tgfb1

gapdh

in-vivo

ablim

h2afz

tgfb1

ablim

sptbn

KSOM

6

ywhaz

18 s

ywhaz

18 s

ywhaz

CZB

sptbn

sptbn

wrnip

sdha

sdha

M16

18 s

gapdh

gapdh

gapdh

sdha

in-vivo

h2afz

actb

sptbn

sdha

gapdh

KSOM

7

sptbn

ywhaz

wrnip

gapdh

wrnip

CZB

gapdh

wrnip

sptbn

sptbn

18 s

M16

tgfb1

wrnip

tgfb1

wrnip

wrnip

in-vivo

actb

tgfb1

ablim

tgfb1

sdha

KSOM

8

actb

sdha

sdha

tgfb1

tgfb1

CZB

ywhaz

gapdh

gapdh

18 s

tgfb1

M16

gapdh

18 s

wrnip

18 s

18 s

in-vivo

sdha

sdha

sdha

sptbn

tgfb1

KSOM

9

gapdh

actb

18 s

sdha

gapdh

CZB

ablim

ablim

ablim

ablim

ablim

M16

ablim

ablim

ablim

actb

ablim

in-vivo

gapdh

ablim

wrnip

actb

ablim

KSOM

10

sdha

gapdh

actb

actb

sdha

CZB

tgfb1

tgfb1

tgfb1

gapdh

gapdh

M16

actb

actb

actb

ablim

actb

in-vivo

wrnip

wrnip

actb

wrnip

wrnip