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Table 5 Statistics of 1, 3 and 5 Gb assemblies of latex and leaf reads

From: RNA sequencing read depth requirement for optimal transcriptome coverage in Hevea brasiliensis

k-mer size Latex 1 Gb Latex 3 Gb Latex 5 Gb Leaf 1 Gb Leaf 3 Gb Leaf 5 Gb
N50 (bp) Total transcripts N50 (bp) Total transcripts N50 (bp) Total transcripts N50 (bp) Total transcripts N50 (bp) Total transcripts N50 (bp) Total transcripts
51 706 64,691 1016 89,882 1053 108,246 544 90,842 831 141,047 845 170,274
53 716 61,113 1058 85,854 1135 104,493 535 84,656 888 129,418 920 158,195
55 724 56,456 1093 79,303 1187 95,718 523 77,815 915 117,125 999 141,823
57 717 52,339 1102 73,610 1231 88,114 517 70,653 933 105,513 1049 127,329
59 707 48,151 1108 67,662 1266 80,620 509 64,286 937 95,662 1079 113,686
61 683 43,857 1100 62,627 1281 73,298 500 58,367 933 86,244 1086 100,912
63 668 40,003 1090 57,318 1270 67,227 490 52,086 912 78,666 1084 90,894
65 657 36,366 1067 53,173 1266 61,105 478 46,805 885 72,173 1059 82,529
67 642 32,702 1041 49,168 1241 55,834 466 41,495 855 66,289 1026 75,410
69 629 29,326 1001 46,360 1201 52,592 451 36,757 810 61,116 988 68,544
71 610 26,079 956 43,095 1151 49,302 439 32,167 761 56,152 944 63,072
73 591 23,055 907 40,051 1103 45,844 432 27,547 713 51,241 879 58,710
75 578 20,055 850 36,849 1052 42,945 427 23,246 669 46,727 823 53,571
77 567 17,326 815 33,398 990 39,996 427 19,079 631 41,838 763 49,712
  1. The optimized N50 length for each tissue assembly is highlighted in bold italics.