Skip to main content
Figure 1 | BMC Research Notes

Figure 1

From: Leishmania braziliensis and Leishmania amazonensis amastigote extracts differ in their enhancement effect on Leishmania infection when injected intradermally

Figure 1

Effect of intradermal injection of L. amazonensis (LaE) and L. braziliensis (LbE) extracts in the infection of BALB/c mice by L. braziliensis . A and C, lesion sizes in groups of five to eight BALB/c mice injected with saline and different doses of LaE (A) or LbE (C). B and D, number of parasites per lesion as estimated by limiting dilution, at the sixth week after infection, of the same mice whose lesion sizes are depicted in A and C, respectively. The animals were treated with a single intradermal injection of saline or of the doses of extracts indicated above the graphs in A and C or below the X axis in B and D. One week later they were infected with 107L. braziliensis promastigotes in the stationary phase of growth. The lines in A and C join the median values of the results from each group in each week, and the vertical bars represent the interquartile interval. Each symbol in B and D represents the result obtained from a single animal. The results of two independent experiments are shown. Symbols and lines corresponding to the results from one experiment are dark blue and the lines are broken; from the other experiment the symbols and lines are red and the lines are continuous. Comparisons between the groups of extract-injected and the group of saline-injected mice were performed by Mann–Whitney U test [level of significance when comparing lesion sizes (A and C) = 0.0056; when comparing lesion parasitism (B and D) = 0.0167]. *P < 0.005; **P < 0.001.

Back to article page