Step-by-step approach for harvesting the sciatic nerve. Mouse preparation and euthanasia (a). Exposure of hind limb muscles and posterior abdominal wall (b-e). Exposure of the sciatic nerve by pulling method (f, g). Tracing and harvesting the sciatic nerve (h, i). APV = Abdomino-pelvic viscera; BHL = Bent hind limb; HL = Hind limb; PAW = Posterior abdominal wall; SN = Sciatic nerve; T = Tail; UO = Urethral opening; XP = Xiphoid process; White dotted line = incision lines; Black dotted lines = small cut.