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Table 2 Comparison of candidate reference genes expression stability using the comparative delta-Ct method

From: Identification of a set of endogenous reference genes for miRNA expression studies in Parkinson’s disease blood samples

Combination

Mean delta-Ct

SD

Mean SD

RNU24 vs Z30

-1.53

0.58

0.93

RNU24 vs RNU6B

-2.26

1.09

 

RNU24 vs miR-103a-3p

-0.71

1.12

 

Z30 vs RNU24

1.53

0.58

0.93

Z30 vs RNU6B

-0.73

0.99

 

Z30 vs miR-103a-3p

0.82

1.23

 

RNU6B vs Z30

0.73

0.99

1.25

RNU6B vs RNU24

2.26

1.09

 

RNU6B vs miR-103a-3p

1.62

1.66

 

miR-103a-3p vs Z30

-0.82

1.23

1.34

miR-103a-3p vs RNU24

0.71

1.12

 

miR-103a-3p vs RNU6B

-1.62

1.66

 
  1. Mean delta-Ct = Ct target – Ct reference; SD = standard deviation of the mean; mean SD = mean of the calculated standard deviations.
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BMC Research Notes

ISSN: 1756-0500

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