Figure 1
From: Cold stress alters transcription in meiotic anthers of cold tolerant chickpea (Cicer arietinum L.)
Flow-chart showing the procedure to generate gene expression profiles. DDRT-PCR was used to study differential transcript expression in cold-stressed chickpea anthers. The genotype used was ICC16349 (cold-tolerant) and the cold treatment was at 5 ± 1°C. Semi-quantitative RT-PCR was used to study temporal and spatial expression of selected genes. The cold stress treatment for DDRT-PCR included two time points (0 and 72 h). Temporal expression in anthers was studied at six time-points (0, 1.5, 12, 24, 72 and 120 h) and spatio-temporal expression at four time points (1.5, 12, 24, and 72 h). The organs used for spatio-temporal expression were anthers, gynoecium, leaves and roots.