Table 2 Functional characterization of 35 differentially expressed transcripts from the DDRT-PCR

Transporter activity genes

A3*

Cation/H⁺ antiporter 14, Arabidopsis thaliana, AT1G06970, 4.1e-05

Ion transport

Antiporter activity

Membrane

A20*

Cation efflux system protein, Agrobacterium radiobactor K84, YP_002543585.1, 0.33

Ion transport, transmembrane transport

Copper ion binding

Membrane

A82-1*

Heavy metal efflux pump CzcA, Gamma proteobacterium, ZP_05061697.1, 4e-03

Unknown

Cation transmembrane transporter activity

Unknown

A116-3*

L-ascorbate oxidase like protein, M. truncatula, XP_003611827.1, 4e-05

Ion transport

Copper ion binding

Unknown

A121-1*

AT5G57110 (Ca2+ transporting ATPase), A. thaliana, BAH20100.1, 6e-05

Calcium transport, ATP biosynthetic process

Calcium ion transport

Membrane

A123-1*

Potassium channel tetramerization domain-containing protein, R. communis, XP_002509821.1, 3e-29

Ion transport

Voltage-gated potassium channel activity

Membrane

A125-1*

F16A14.19, A. thaliana, AAF79412.1, 8e-20

Transport

Anion channel activity

Unknown

A118**

ABC transporter family, M. truncatula, XP_003590459.1, 8e-27

Unknown

Ttransporter activity

Plasmodesmata

Translation

A22*

40S ribosomal protein SA, M. truncatula, XP_003638087.1, 4e-3

Translation

Ribonucleoprotein

Cytoplasm

A73**

60S ribosomal protein L27a-3, M. Truncatula, XP_003613127.1, 2e-16

Translation

Structural constituent of ribosome

Ribosome

AC39GA2*

Translation initiation factor EIF-2B epsilon, M. truncatula, XP_003618849.1, 3e-06

Translation

Translation initiation factor activity

Unknown

AC41GF1**

60S ribosomal protein L34, M. truncatula, XP_003621181.1, 6e-09,

Translation

Ribnucleoprotein

Large subunit of ribosome

Transcription regulator

AC 47G E1*

SRCI, Glycine max, BAA19768.1, 0.096

Cold stress regulation

Transcription

Unknown

Carbohydrate metabolism

A36-2*

Beta-galactosidase, Arabidopsis thaliana, CAB64750.1, 4e-3

Carbohydrate metabolism, pollen development

Beta-galactosidase activity

Apoplast

A59-2*

Glycerol kinase, Glycine max, NP_001237303.1, 1e-21

Glycerolipid metabolism

Glycerol kinase activity

Unknown

A102-2*

Aconitate hydratase, M. truncatula, XP_003612247.1, 4e-24

Carbohydrate metabolism (converts citrate to isocitrate)

Iron sulfur cluster binding

Cytoplasm

AC44GA 2*

Sucrose phosphorylase, Vibrio harveyi HY01, ZP 01985256.1, 0.64

Starch and sucrose metabolism

Cation binding, sucrose phosphorylase activity

Unknown

Pollen development

A10*

Peroxisomal ABC transporter, M. truncatula, XP_003601968.1, 1e-10

Transport (fatty acids), Pollen tube elongation, ovule fertilization, and seeds germination after imbibition

ATP binding

Glyoxisomal membrane

A60*

Pectin methylesterase, M. truncatula, XP_003595372.1, 7e-17,

Cell wall modification, tetrad separation, pollen tube growth

Pectin methylesterase activity

Membrane

A99-1*

Microspore-specific promoter2, Arabidopsis thaliana, NP_5686669.1, 0.02

Pollen development

Transcription

Chloroplast

A101*

Pectin esterase, M. truncatula, XP_003591164.1, 1e-06

Cell wall modification, pollen tube growth

Pectin methylestera activity

Cell wall

A104-2*

SYP124 (SYNTAXIN OF PLANTS); SNAP receptor, M. truncatula, XP_003593444.1, 1e-3

Vesicular mediate transport, intracellular protein transport, pollen development

SNAP receptor activity

Membrane

AC52GD1*

Protein WAX2, M. truncatula, XP_003606194.1, 5e-28

Pollen sperm cell differentiation

Iron ion binding, fatty acid biosynthetic process

Integral to membrane

A64-1**

Early nodulin-like protein, M. truncatula, XP_003609073.1, 8e-04

Pollen development

Copper ion binding

Membrane

Signal transducer activity

A67*

Cysteine-rich receptor-like protein kinase, M. truncatula, XP_003589476.1, 2e-3

Calcium-mediated Signal transduction, pollen development, recognition of pollen

Protein serine/threonine kinase activity

Membrane

A81*

Protein kinase serine/threonine, A. thaliana, CAA16700.1, 1e-37

Signal transduction

Protein serine/threonine kinase activity

Nucleus

A97-2*

Ralf-like 19 protein, , A. thaliana, NP_850219.1, 5e-25

Signal transduction

Unknown

Unknown

A120-2*

Serine/threonine protein kinase, M. truncatula, XP_003618563.1, 2e-3

Signal transduction

Protein serine/threonine kinase Activity

Unknown

A140-2* (pollen development)

Cyclin-dependent kinase CDC2C, M. truncatula, XP_003621316.1, 2e-36

Signal transduction, pollen tube growth

Serine/threonine protein kinase

Unknown

AN59CA2*

Casein kinase, Ricinus communis, XP_002516524.1, 5e-17

Signaling transduction

ATP binding

Unknown

Defense

A126-1*

Wound responsive protein, Phaseolus vulgaris, Q09020.1, 7e-07

Defense

Unknown

Unknown

A114**

RRP1, Medicago truncatula, AB1511616.1, 0.1e-4

Defense, resistance to Peronospora parasitica

Unknown

Unknown

Energy

AC45GA3**

ATPase subunit 8, Lotus japonicus, YP_005090498.1, 2e-71

Energy

Hydrogen ion transmembrane transporter activity

Mitochondria

Metabolic processes

A98-2*

Hydrolase, Zea mays, NP_001150070.1, 1e-6

Unknown

Hydrolase activity

Unknown

Cell division

A71-1**

Cell division cycle and apoptosis regulator protein, M. truncatula, XP_003613873.1, 5e-07

Cell division

Unknown

Unknown