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Figure 2 | BMC Research Notes

Figure 2

From: The cytological and molecular role of DOMAINS REARRANGED METHYLTRANSFERASE3 in RNA-dependent DNA methylation of Arabidopsis thaliana

Figure 2

Bisulfite sequencing analysis at distinct RdDM targets in drm3 mutant. (A) Frequency of cytosine methylation according to sequence context at MEA-ISR loci. A significant reduction of methylation at non-CG sites was observed in drm3 relative to WT, but not to the levels observed for drm2. Frequency of symmetrical site methylation at (B) AtSNI and (C) siRNA02 (CG) are differentially affected by drm2 and drm3. (D) Loss of CHH methylation at IGN5 is higher in a drm2 background relative to drm3, especially at its 5’ end (E). All cytosines are in a CHH context except at positions 101 (CHG) and 109 (CG) (E). Where present, error bars refer to standard deviation. y-axis: frequency (%); x-axis: numbers correspond to cytosine position.

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