Figure 4

DRM3 regulates NOR interphase organization. (A) DNA FISH showing decondensation of 45S rRNA loci ( red ) in drm3 and drm2 mutants. Unlike met1, drm3 is not required for chromocenter organization, as observed by WT interphase distribution of (B) CEN repeats (green), 5S rRNA loci (red) and (C) H3K9me2 in a drm3 background. Representative images are shown for each genotype, corresponding to frequencies >91 %. (n > 64). DNA visualized with DAPI (grey). Size bar = 5 μm.