Representation of an mPCR/RLB of 16 gene targets of 16 Bordetella pertussis isolates. Positive signals were interpreted as the presence of a gene, and no signal was interpreted as absent genes. IS481 was used as a positive control for the 5-plex mPCR and Tohama I was used to ensure probe binding. The amplification of IS481 and targets in Tohama I were always present. Genes that did not show a signal with Tohama I were excluded from analysis. If any isolate strain did not produce a positive signal for IS481, the mPCR was repeated.