From: Effect of RNA integrity on uniquely mapped reads in RNA-Seq
Protocol | RIN | R to RIN 9.4 sample | Total Reads |
---|---|---|---|
NuGEN Ovation RNA-Seq FFPE System + Illumina TruSeq DNA Sample Preparation | 9.4 | 1 | 37,959,903 |
7.4 | 0.971 | 51,131,950 | |
5.3 | 0.967 | 32,285,466 | |
4.5 | 0.955 | 58,476,532 | |
Epicentre RiboZero rRNA Removal Kit + Illumina TruSeq DNA Sample Preparation | 9.4 | 1 | 25,339,734 |
7.4 | 0.958 | 59,722,682 | |
5.3 | 0.956 | 48,134,260 | |
4.5 | 0.951 | 50,650,245 | |
Illumina TruSeq RNA Sample Preparation | 9.4 | 1 | 59,509,601 |
7.4 | 0.984 | 35,262,055 | |
5.3 | 0.533 | 20,939,261 | |
 | 4.5 | 0.366 | 4,531,318 |