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Table 1 R between degraded sample and intact sample for each protocol

From: Effect of RNA integrity on uniquely mapped reads in RNA-Seq

Protocol

RIN

R to RIN 9.4 sample

Total Reads

NuGEN Ovation RNA-Seq FFPE System + Illumina TruSeq DNA Sample Preparation

9.4

1

37,959,903

7.4

0.971

51,131,950

5.3

0.967

32,285,466

4.5

0.955

58,476,532

Epicentre RiboZero rRNA Removal Kit + Illumina TruSeq DNA Sample Preparation

9.4

1

25,339,734

7.4

0.958

59,722,682

5.3

0.956

48,134,260

4.5

0.951

50,650,245

Illumina TruSeq RNA Sample Preparation

9.4

1

59,509,601

7.4

0.984

35,262,055

5.3

0.533

20,939,261

 

4.5

0.366

4,531,318