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Figure 4 | BMC Research Notes

Figure 4

From: Expression level of a flavonoid 3′-hydroxylase gene determines pathogen-induced color variation in sorghum

Figure 4

Results of mapping of short reads by using mRNA-seq at the F3′H locus. Reads from Nakei-MS3B (Nakei) and #96 were mapped on the Nakei-MS3B genome, which has two flavanone 3′-hydroxylase (F3′H) genes (Sb04g024710N and Sb04g024750) because of the genomic deletion. Reads from lines #62, #3, and M36001 were mapped on the BTx623 genome, which has three F3′H genes (Sb04g024710, Sb04g024730, and Sb04g024750). Line #62 has heterozygous alleles, but only the allele derived from M36001 is shown. F3′H expression was strongly induced in sorghum lines with purple lesions (Sb04g024710N: Nakei and #96) and intermediately in the line with red lesions (Sb04g024710: #62), but it was barely expressed in those with orange lesions (Sb04g024710: #3) or no color change (Sb04g024710: M36001). Arrows indicate transcription from the unannotated region between Sb04g024710 and Sb04g024730.

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