Table 1 Gene specific primers used for qPCR
Gene/transcript isoform | Accession number | Sequence | Amplicon size |
|---|---|---|---|
eef1a1l1 (F) | NM_131263.1 | 5′-CTGGAGGCCAGCTCAAACAT-3′ | 87 bp |
eef1a1l1 (R) | 5′-ATCAAGAAGAGTAGTACCGCTAGC-3′ | ||
tra2b (F) | NM_201197 | 5′-GCAGACGACATATTGGTGACC-3′ | 155 bp |
tra2b (R) | 5′-TGACTGCTGGTCGTACACAATG-3′ | ||
maptb 4R (F) | XM_005171601 | 5′-AAGATCGGCTCCACTGAGAACC-3′ | 194 bp |
maptb 4R (R) | 5′-GATCCAACCTTTGACTGGGCTT-3′ | ||
maptb 3R (F) | XM_005171601 | 5′-GGGAAGGGGTGGAAATGTC-3′ | 140 bp |
maptb 3R (R) | 5′-GATCCAACCTTTGACTGGGCTT-3′ | ||
mapta 6R (F) | XM_001340530 | 5′-TCGTCACAAACCAGGTGGAG-3′ | 152 bp |
mapta 6R (R) | 5′-GCTCACGGAACGTCAGTTTG-3′ | ||
mapta 4R (F) | XM_001340530 | 5′-CGGAGGTGGAAAATTGAGTCAC-3′ | 100 bp |
mapta 4R (F) | 5′-CTCCTCCAGGGACACAATTTCT-3′ | ||
maptb −3 (F) | XM_005171601 | 5′-GAAGCCAAGGCTGGAGCA-3′ | 120 bp |
maptb −3 (R) | 5′-CTGGGGATGCCTGTGACTGA-3′ | ||
maptb +3 (F) | XM_005171601 | 5′-CCGGCAACAACATAGCATCTG-3′ | 140 bp |
maptb +3 (R) | 5′-CACCGGGAGTGAATGTGGC-3′ | ||
mapta Ex.6 (F) | XM_001340530 | 5′-CCTAAATCTCCTGCCAGCAAG-3′ | 117 bp |
mapta Ex.6 (R) | 5′-TGTGGGCGAACGGTTCTT-3′ | ||
maptb Ex.6 (F) | XM_005171601 | 5′-CAAATCACCTGGCTCGCTG-3′ | 114 bp |
maptb Ex.6(R) | 5′-GGTTGGTGTTTGAGGTTCTCAGTG-3′ |